mafa antibody  (Bio-Techne corporation)

Journal: bioRxiv

Article Title: Tirzepatide improves pancreatic β-cell function in mice and patients with type 2 diabetes

doi: 10.64898/2026.02.13.704343

Figure Lengend Snippet: ( A ) Representative confocal images of islets by immunofluorescence staining from control group, HFD + vehicle, and HFD + 10 nM [D-Ala2]-GIP, HFD + 10nM semaglutide, HFD + 3 nM tirzepatide, HFD + 10 nM tirzepatide. Endocrine cells were labeled with insulin (red), glucagon (green), and ALDH1A3 (white). The nuclei were stained with Dapi (blue). The insets (dash box) of each group are magnified in the right panels of the images. ( B ) Quantification of relative levels of ALDH1A3 in each group, mice n ≥ 3. ( C ) Representative confocal images of islets by immunofluorescence staining from each group. Endocrine cells were labeled with insulin (red), glucagon (green), and PDX1 (white). The nuclei were stained with Dapi (blue). The insets (dash box) of each group are magnified in the right panels of the images. ( D ) Quantification of relative levels of PDX1 in each group, mice n ≥ 4. ( E ) Representative confocal images of islets by immuofluorescence staining from each group. Endocrine cells were labeled with insulin (red), glucagon (green), and MAFA (white). The nuclei were stained with Dapi (blue). The insets (dash box) of each group are magnified in the right panels of the images. Scale bar indicates 20 μm. ( F ) Quantification of relative levels of MAFA in each group, mice n ≥ 4. Data are presented as mean ± SEM, with significance determined by one-way ANOVA compared to the Vehicle group: * p < 0.05, ** p < 0.01 and **** p < 0.0001.

Article Snippet: Primary antibodies against GAPDH, AKT1, p-AKT(Ser473), FOXO1 were from Proteintech (GAPDH, 60004-1-Ig; AKT1, 10176-2-AP, p-AKT (Ser473), 66444-1-Ig, FOXO1, 18592-1-AP); antibodies against p-FOXO1(Ser256), PDX1, MAFA, and ALDH1A3 were from Affinity (AF3417), Abcam (Ab219207), Abcam (Ab17976) and Novus Biologicals (NBP2-15339), respectively.

Techniques: Immunofluorescence, Staining, Control, Labeling

Journal: bioRxiv

Article Title: Tirzepatide improves pancreatic β-cell function in mice and patients with type 2 diabetes

doi: 10.64898/2026.02.13.704343

Figure Lengend Snippet: ( A ) Schematic workflow of single-cell RNA sequencing of mouse islets. ( B-C ) t-SNE plots showing all cells colored by cell type ( B ) and sample origin ( C ). ( D ) Proportions of different cell types among the three sample origins. ( E ) Expression levels of Pdx1 and Mafa in β cells from the three origins. ( F ) Identification of common differentially expressed genes using three machine learning methods. ( G-H ) Gene set enrichment analysis (GSEA) of FOXO pathway related genes in β cells comparing the HFD and CTR groups ( G ), as well as the TZP and HFD groups ( H ).

Article Snippet: Primary antibodies against GAPDH, AKT1, p-AKT(Ser473), FOXO1 were from Proteintech (GAPDH, 60004-1-Ig; AKT1, 10176-2-AP, p-AKT (Ser473), 66444-1-Ig, FOXO1, 18592-1-AP); antibodies against p-FOXO1(Ser256), PDX1, MAFA, and ALDH1A3 were from Affinity (AF3417), Abcam (Ab219207), Abcam (Ab17976) and Novus Biologicals (NBP2-15339), respectively.

Techniques: Single Cell, RNA Sequencing, Expressing

Journal: Genetics, Selection, Evolution : GSE

Article Title: A 3′ UTR polymorphism g.1618 G > A in the MAFA gene modulates miR-3678-3p binding and enhances meat production in sheep via the MAFA/GHR/JAK2 pathway

doi: 10.1186/s12711-025-01024-7

Figure Lengend Snippet: The transcription factor MAFA targets the GHR gene to promote muscle growth. a Venn diagram showing the overlap between MAFA target genes identified by ChIP-seq and differentially expressed genes from transcriptome analysis. b ChIP-seq peak profile indicating binding of MAFA to the GHR gene. c Heatmap showing Pearson correlation coefficients among expression levels of GHR and MAFA (FPKM values) and the proportion of longissimus dorsi muscle (n = 10). d Western blot analysis validating the chromatin immunoprecipitation (ChIP) efficiency of MAFA. Input: Cell lysate after centrifugation; SN: Supernatant after immunoprecipitation; IP: Antibody-magnetic bead complex after incubation and washing; IgG: IgG-magnetic bead complex after incubation and washing. e ChIP-PCR results showing significant enrichment of the GHR gene by MAFA (n = 3). f – h Western blot analysis of GHR expression and JAK2 phosphorylation levels in the longissimus dorsi muscle of individuals with different genotypes (n = 3, same batch of samples as in Fig. e, with shared β-actin internal control). Data are presented as mean ± SEM. Statistical significance is indicated as follows: ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.005; **** P < 0.001; ***** P < 0.0005

Article Snippet: Immunoprecipitation was performed using an anti-MAFA antibody (#79,737, Cell Signaling Technology, USA), and IgG served as a negative control.

Techniques: ChIP-sequencing, Binding Assay, Expressing, Western Blot, Chromatin Immunoprecipitation, Centrifugation, Immunoprecipitation, Incubation, Phospho-proteomics, Control